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Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 230-236 doi: 10.15302/J-FASE-2015071

摘要： Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion chromatography (SEC), and were compared with particles from ultracentrifugation by transmission electron microscopy (TEM), infectivity and recovery test, and real time fluorescent quantitative PCR (FQ-PCR). TFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system. CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0×10 TCID ·mL to 3.0×10 TCID ·mL , but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone. In addition, pure CSFV particles with the expected diameter of 40–60 nm were roughly spherical without any visible contamination. Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV ( <0.05). The purification procedures in this study are reliable technically and feasible for purification of large volumes of viruses.

关键词： classical swine fever virus     virus purification     tangential-flow filtration     size-exclusion chromatography    

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Enhanced cross-flow filtration with flat-sheet ceramic membranes by titanium-based coagulation for membrane

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1531-x

摘要：

• Ceramic membrane filtration showed high performance for surface water treatment.

关键词： Ceramic membrane     Coagulation     Polytitanium chloride     Membrane fouling    

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Characterization of particle deposition during crossflow filtration as influenced by permeate flux andcrossflow velocity using a microfluidic filtration system

Hongzhan Di, Gregory J. O. Martin, Dave E. Dunstan

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 552-561 doi: 10.1007/s11705-020-1962-5

摘要： Particle deposition during crossflow filtration is significantly influenced by the operating conditions, in particular the permeate flux and crossflow velocity. However, there is a lack of detailed knowledge about how deposit layer structures and distributions depend on operating parameters. This study uses a microfluidic visualisation filtration system to examine the influence of operating conditions on the deposition process during crossflow ultrafiltration from a microscopic perspective. Increasing the permeate flux caused an increasing amount of deposition and a thicker deposit layer. Higher crossflow velocities reduced the extent of deposition. The degree of deposition varied over a range of operating conditions due to the altered hydrodynamic forces exerted on the particles, which can be examined by the deposition probability according to an existing model. Building on this, an empirical correlation between the deposition probability and volume of deposition as function of filtration time was developed, which gave good agreement with experimental results. The effect of solution conditions was also involved in this correlation as a interaction energies. This could be useful for predicting the dynamic deposition process during crossflow filtration over a range of operating and solution conditions.

关键词： particle deposition     crossflow filtration     microfluidics     confocal microscopy    

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Removal of virus aerosols by the combination of filtration and UV-C irradiation

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1627-y

摘要：

● The removal of virus aerosols by filtration and UV-C irradiation was proposed.

关键词： Filtration system     UV-C irradiation     Virus aerosol     Public health     COVID-19    

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不同立杆高度毛竹材弦切面的润湿性能研究

张巧玲,周洪伟,梁廷斌,仝雪艳,曾钦志

《中国工程科学》 2014年 第16卷 第4期   页码 56-59

摘要：

本文研究了H2O、酚醛树脂胶黏剂（PF）、脲醛树脂胶黏剂（UF）和改性大豆蛋白胶（MSA）在毛竹材不同立杆高度弦切面的润湿性能，通过测定这些润湿液体在竹材弦切面上的接触角，建立润湿液体在毛竹材弦切面的动态润湿模型，分析毛竹材的不同高度对润湿液体动态润湿性能的影响，并对比分析各种润湿液体在毛竹材弦切面的润湿性能差异。

关键词： 毛竹     立杆高度     弦切面     接触角     润湿性    

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Membrane fouling control by ultrasound in an anaerobic membrane bioreactor

SUI Pengzhe, WEN Xianghua, HUANG Xia

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 362-367 doi: 10.1007/s11783-007-0062-9

摘要： In this study, ultrasound was used to control the membrane fouling online in an anaerobic membrane bioreactor (AMBR). Short-term running experiments were carried out under different operating conditions to explore feasible ultrasonic parameters. The experimental results indicated that when the crossflow velocity was more than 1.0 m/s, membrane fouling could be controlled effectively only by hydrodynamic methods without ultrasound. When ultrasound was applied, an ultrasonic power range of 60–150 W was suitable for the membrane fouling control in the experimental system. The experimental results showed that the membrane fouling was controlled so well that membrane filtration resistance (Σ) could stay at 5 × 10 m for more than a week with the crossflow velocity of 0.75 m/s, which equaled the effect of crossflow velocity of more than 1.0 m/s without ultrasound.

关键词： membrane filtration     filtration resistance     different     feasible ultrasonic     anaerobic membrane    

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树盘干缩异向性引起应变的测算及分析

付宗营,赵景尧,蔡英春

《中国工程科学》 2014年 第16卷 第4期   页码 25-29

摘要：

研究探讨了干燥过程中树盘受抑制干缩应变、自由干缩应变、弹性应变、黏弹性蠕变应变以及机械吸附蠕变的图像解析测算法；运用该方法测算了白桦树盘常规缓慢干燥（含水率分布均匀）过程中干缩异向性引起的弦向各应变，分析了干燥过程中不同含水率阶段的应力状态及应力与各应变的关系。结果表明：应变的图像解析测算法可满足精度要求；树盘干燥至fiber saturation point( FSP)以下，弦向首先受拉伸应力作用，随着干燥的进行，拉伸应力转变为压缩应力；应力方向与各应变对应关系不同，与黏弹性蠕变应变无明显对应关系，与机械吸附蠕变基本对应。

关键词： 图像解析法     树盘     干缩异向性     干燥应力     弦向应变    

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and computational assessment of 1,4-Dioxane degradation in a photo-Fenton reactive ceramic membrane filtration

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1341-y

摘要：

• 1,4-Dioxane was degraded via the photo-Fenton reactive membrane filtration.

关键词： Photo-Fenton     Ceramic membrane     1     4-Dioxane     Goethite    

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面向未来饮用水净化的OSRO技术——基于河岸过滤的一步反渗透技术 Perspective

Yujia Zhai, 刘刚, Walter G.J. van der Meer

《工程（英文）》 2022年 第9卷 第2期   页码 27-34 doi: 10.1016/j.eng.2021.02.015

摘要： 本文介绍了一种由河岸过滤（riverbank filtration, RBF）和反渗透（reverse osmosis, RO）组成的一步反渗透（one-step reverse osmosis, OSRO人工滤池（artificial bank filtration, ABF）与砂滤系统相结合，起到人工补给的作用，是OSRO概念中RBF的替代方案，用于从当地可用资源中供应饮用水。

关键词： 饮用水处理     河岸过滤     反渗透     人工滤池     水资源回收和再利用    

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Polyhedral linkages obtained as assemblies of planar link groups

GÖkhan KİPER, Eres SÖYLEMEZ

《机械工程前沿（英文）》 2013年 第8卷 第1期   页码 3-9 doi: 10.1007/s11465-013-0363-6

摘要：

The study aims to devise means of obtaining polyhedral linkages for homothetic deployment of polyhedral shapes by embedding planar link groups in faces of the polyhedral shape of interest. The questions of which polyhedral shapes may be suitable for such a purpose and what are the compatibility conditions for spatially assembling planar link groups are addressed. Homohedral and tangential polyhedral shapes are found to be suitable for the task and some examples of linkages are worked out.

关键词： polyhedral linkages     homothety     homohedra     tangential polyhedra    

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长纤维高速过滤器的深层过滤过程与特性

王世和,周飞,鄢璐

《中国工程科学》 2009年 第11卷 第2期   页码 43-47

摘要：

以自行开发的长纤维高速过滤器为对象，通过在净水厂长期的过滤试验，从过滤器床层孔隙分布、 床层内滤液浓度及各层比积泥量和水头损失沿滤层的变化等方面，详细分析了长纤维高速过滤器基于结构 上独特特点而形成的积泥逐层向滤床深处推移，直至整个滤床“衰竭”的典型深层过滤过程与特征，为过滤技 术的深度开发提供了依据。

关键词： 长纤维高速过滤器     深层过滤     过程机理    

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various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes in glomerular filtration

Seo Yeon Baik, Hyunah Kim, So Jung Yang, Tong Min Kim, Seung-Hwan Lee, Jae Hyoung Cho, Hyunyong Lee, Hyeon Woo Yim, Kun-Ho Yoon, Hun-Sung Kim

《医学前沿（英文）》 2019年 第13卷 第6期   页码 713-722 doi: 10.1007/s11684-018-0661-9

摘要： Few long-term follow-up studies have compared the changes in renal function according to the type of statin used in Korea. We compared the long-term effects of statin intensity and type on the changes in the glomerular filtration rate (GFR). We extracted data of patients who took statin for the first time. We analyzed whether or not different statins affect the changes in GFR at 3 months after baseline and 4 years after. We included 3678 patients and analyzed the changes in GFR. The GFR decreased by 3.2%±0.4% on average 4 years after the first statin prescription, indicating statistically significant deterioration (from 83.5±0.4 mL/min/1.73 m to 79.9±0.4 mL/min/1.73 m , <0.001). When comparing the GFR among different statins, significant differences were observed between atorvastatin and fluvastatin (−5.3%±0.7% vs. 1.2%±2.2%, <0.05) and between atorvastatin and simvastatin (−5.3%±0.7% vs. −0.7%±0.8%, <0.05). In pitavastatin (odds ratio [OR]=0.64, 95% confidence interval [CI]=0.46–0.87, <0.005) and simvastatin (OR=0.69, 95% CI=0.53–0.91, <0.008), the GFR rate that decreased by<60 mL/min/1.73 m was significantly lower than that of atorvastatin. Regarding long-term statin intake, GFR changed with the type of statin. This work is the first in Korea to compare each statin in terms of changes in the GFR after the statin prescription.

关键词： statin     glomerular filtration rate     HMG-CoA reductase inhibitor     chronic kidney disease    

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Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 327-335 doi: 10.1007/s11783-014-0763-9

摘要： A novel method was applied to co-recover proteins and humic acid from the dewatered sewage sludge for liquid fertilizer and animal feed. The proteins in sewage sludge were first extracted using the processes of ultra-sonication and acid precipitation, and then the humic acid was recovered via membrane filtration. The extraction efficiency was 125.9 mg humic acid?g VSS volatile suspended solids (VSS) and 123.9 mg proteins?g VSS at the optimal ultrasonic density of 1.5 W?mL . FT-IR spectrum results indicated that the recovered proteins and humic acid showed similar chemical characteristic to the natural proteins and humic acid. The acidic solution (pH 2) could be recycled and used more than 10 times during the co-recovery processes. In addition, the dewatered sludge could be easily biodegraded when the humic acid and proteins are extracted, which was essential for further utilization. These findings are of great significance for recovering valuable nutrient from sewage sludge.

关键词： sewage sludge     co-recovery     proteins     humic acid     recycling     biodegradation rate    

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Numerical study of internal flow field and flow passage improvement of an inlet particle separator

Florian PAOLI, Tong WANG

《能源前沿（英文）》 2011年 第5卷 第4期   页码 386-397 doi: 10.1007/s11708-011-0156-8

摘要： By performing gas flow field numerical simulations for several inlet Reynolds numbers (from 2 × 10 to 9 × 10 ) and byflow ratios (from 10% to 20%), the present study has proposed to improve the flow passage of an inlet particle separator. An adjacent objective of the study is to lower pressure losses of the inlet particle separator (IPS). No particle has been included in the gas flow for a -epsilon turbulence model. The velocity distribution in different sections and the pressure coefficient along the duct have been analyzed, which indicates that there exist important low-velocity regions and vortices in the separation area. Therefore, the profile of streamlines along the original passage has been considered. This profile illustrated a vacuum region in the same area. All investigations suggest that the separation area is the most critical one for fulfilling the objective on pressure losses limitation. Then the flow passage improvement method has focused on the separation area. An improved shape has been designed in order to suit smoothly to the streamlines in this region. Similar numerical studies as those for the original shape have been conducted on this improved shape, confirming some considerable enhancements compared with the original shape. The significant vortices which appear in the original shape reduce in amount and size. Besides, pressure losses are greatly decreased in both outlets (up to 30% for high Reynolds number) and the flow is uniform at the main outlet. Subsequent engineering surveys could rely on expressions obtained for in both outlets which extend the pressure losses for a wide range of inlet Reynolds numbers. As a result, the numerical calculations demonstrate that the flow passage improvement method applied in this study has succeeded in designing a shape which enhances the flow behavior.

关键词： streamlines     pressure losses     flow passage improvement     inlet particle separator (IPS)    

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G-CNTs/PVDF mixed matrix membranes with improved antifouling properties and filtration performance

Xiaoyan Guo, Chunyu Li, Chenghao Li, Tingting Wei, Lin Tong, Huaiqi Shao, Qixing Zhou, Lan Wang, Yuan Liao

《环境科学与工程前沿（英文）》 2019年 第13卷 第6期 doi: 10.1007/s11783-019-1165-9

摘要：

A novel nanocomposite OMWCNT-A-GO was synthesized by conjugating OMWCNT and GO.

The P-OMWCNT-A-GO membrane was fabricated by non-solvent induced phase inversion.

The P-OMWCNT-A-GO exhibits the best water flux, BSA rejection and flux recovery.

It should be due to the enhanced membrane pore size, porosity and hydrophilicity.

关键词： carbon nanotubes     graphene oxide     mixed matrix membrane     nanohybrid     antifouling membrane     membrane hydrophilicity    

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标题 作者 时间 类型 操作

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

期刊论文

Enhanced cross-flow filtration with flat-sheet ceramic membranes by titanium-based coagulation for membrane

期刊论文

Characterization of particle deposition during crossflow filtration as influenced by permeate flux andcrossflow velocity using a microfluidic filtration system

Hongzhan Di, Gregory J. O. Martin, Dave E. Dunstan

期刊论文

Removal of virus aerosols by the combination of filtration and UV-C irradiation

期刊论文

不同立杆高度毛竹材弦切面的润湿性能研究

张巧玲,周洪伟,梁廷斌,仝雪艳,曾钦志

期刊论文

Membrane fouling control by ultrasound in an anaerobic membrane bioreactor

SUI Pengzhe, WEN Xianghua, HUANG Xia

期刊论文

树盘干缩异向性引起应变的测算及分析

付宗营,赵景尧,蔡英春

期刊论文

and computational assessment of 1,4-Dioxane degradation in a photo-Fenton reactive ceramic membrane filtration

期刊论文

面向未来饮用水净化的OSRO技术——基于河岸过滤的一步反渗透技术

Yujia Zhai, 刘刚, Walter G.J. van der Meer

期刊论文

Polyhedral linkages obtained as assemblies of planar link groups

GÖkhan KİPER, Eres SÖYLEMEZ

期刊论文

长纤维高速过滤器的深层过滤过程与特性

王世和,周飞,鄢璐

期刊论文

various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes in glomerular filtration

Seo Yeon Baik, Hyunah Kim, So Jung Yang, Tong Min Kim, Seung-Hwan Lee, Jae Hyoung Cho, Hyunyong Lee, Hyeon Woo Yim, Kun-Ho Yoon, Hun-Sung Kim

期刊论文

Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

期刊论文

Numerical study of internal flow field and flow passage improvement of an inlet particle separator

Florian PAOLI, Tong WANG

期刊论文

G-CNTs/PVDF mixed matrix membranes with improved antifouling properties and filtration performance

Xiaoyan Guo, Chunyu Li, Chenghao Li, Tingting Wei, Lin Tong, Huaiqi Shao, Qixing Zhou, Lan Wang, Yuan Liao

期刊论文